STRUM results for single-point mutation job
First step : Please copy and paste your data (Note: STRUM with sequence in FASTA format will run I-TASSER to get 3D structure, which will take more time than that with 3D Structure).
I-TASSER model: either I-TASSER model in PDB format or sequence in FASTA format is acceptable.
Second step : Please input a list of mutations
(one per mutation set per line with single-point mutations set out off by semicolons,
WT residue followed by the position of the mutation followed by the mutated residue). Example,
K1A; -Single mutation Lys, the 1th residue, is mutated to Ala
Download the submitted query structure
Download the predicted ddG of single-point mutation you submited.
Position
Wild-type
mutant type
ddG
1
K
A
0.12
9
L
G
-3.85
9
L
I
-1.1
16
D
K
-0.67
119
H
L
0.41
STRUM results for systematic job
First step : Please copy and paste your data.
I-TASSER model: either I-TASSER model in PDB format or sequence in FASTA format is acceptable.
Second step : Please input a list of segments
(one segment set per line with multi-segments set out off by semicolons,
the stard position followed by "-" followed by the end position). Example,
1-68; -the segment from 1th residue to 136th residue for this protein
Download the predicted 3D model by I-TASSER
Segment 1 from 1th residue to 136th residue.
Download the score file which describes the possibility of mutation for each residue.
Download all the predicted ddGs for each residue mutanted to other 19 possible amino acids.
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0
3.20
6.39
9.59
12.78
15.98
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22.37
25.57
28.77
31.96
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Please cite the following articles when you use the STRUM server:
1.
Lijun Quan, Qiang Lv, Yang Zhang
STRUM: Structure-based prediction of protein stability changes upon single-point mutation.
Boinformatics, 32: 2936-46 (2016).