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How to use the model II: Systematic

  • First step: Please copy and paste your data (Note: STRUM with sequence in FASTA format will run I-TASSER to get 3D structure, which will take more time than that with 3D Structure).
      1) Either sequence in FASTA format is acceptable.
      2) Or experimental resolved structure in PDB format is acceptable.
  • Second step: Please input a list of segments (one segment set per line with multi-segments set out off by semicolons, the stard position followed by "-" followed by the end position). Example,
      10-40; the segment from 10th residue to 40th residue for this protein
      79-128;
      129-129;
  • Last step: You will get the results as shown below.

    • Systematic Results


  • By right-clicking on the images, you can change the configurations, e.g. modifying the background color or stopping the spin of your models.
    Download the predicted 3D model by I-TASSER



  • As shown in the figures below, X axis presents the protein sequence positions, y axis presents a score which describes the possibility of mutation for each residue and the height of the amino acid indicates the possiblity of mutating into the correspongding amino acid type from wild-type residue.
    Download the score file which describes the possibility of mutation for each residue.
    Download all the predicted ddGs for each residue mutanted to other 19 possible amino acids.
    Segment 1 from 10th residue to 40th residue.

    10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 0 2.03 4.06 6.09 8.12 10.15 12.18 14.20 16.23 18.26 20.29 I G D K P E A N S T R H Q W Y F C V L M K D P E W G N H S A F T V R Y I L C Q M A K D E H Q N R P Y W S T G F V I L C M I D G A E P S N T H W K Y Q F C R V L M D W Y F E H I R P L C G M S V Q N A K T G K Y D R E W H F N Q M T P V I L C S A D F Y W I R M H C P V K E L S A Q T G N T D E H G K P W A N Q Y F C I R S L M V V G D K E N H S P Q R T C A W Y M F L I K D P E G W R Y N H S A F T Q C V I L M L D E P G H N Q K S R W A T Y C V F M I M G D S P A E N K H T Q W R C Y V F I L Y P D G E K N Q T S A H R V C I L M F W K D P W Y H E A G L N F V Q C S I T M R G V L T D I N Q W Y H P E F A M S C K R Q K R N D H P W G Y F T E M S A V L I C P N H K G Q D E S R T Y A C W F V M I L M D G P K S A N E H R Q T W Y C F L V I T D G E H K A N Q P Y W R S C F V M L I F G D E P S N K T A H Q R W C Y V M I L R D W G E S F N P Y A T V L K H C I Q M L D P G H E N R S K Q T W Y A C V F M I L D P G E K H R S N T Q W Y A V F C I M L P H K E D A R N W Y Q S C T G F I V M V E D G Y H Q N R K S P W A C T M F L I D Y W H F P E R I G K A C N M S T V Q L T H D E K Q W N Y R G P S F A M L V C I P H K D E Q R N Y W F M G S T A V I L C E W Y H D K N Q P I F S T R G V A C L M T E H K A Y P F G W D Q S R N C I L M V K D W Y H N F Q R T P G S M V E L I C A


    Segment 2 from 79th residue to 128th residue.
    Download the score file which describes the possibility of mutation for each residue.
    Download all the predicted ddGs for each residue mutanted to other 19 possible amino acids.

    79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 0 2.61 5.21 7.82 10.43 13.03 15.64 18.25 20.86 23.46 26.07 K D W Y E H P F C I N Q G S A R L T V M Y D E P N Q G H K A L S R T V M C I W F G K T Y Q N V L F P R I W D M S E H C A R F D E W G Y P S H M N A C I Q K V T L G D E P K N H Q W T R S C A Y L I V M F L D G P E H N S Q R K A W Y T V C F M I A D E H K N Q P Y W R T S G F M C L I V Y G D E N S P R A Q K H T V C L I M F W I D E G P H N S K Q R T Y W A C F M V L Y G D P A E Q S N T V K L C I H M F R W A D E H N P Q G Y W T K S R F M L C I V D F I L Y W M C V H Q R A G K P S T N E G V I L K T D F R E M N Y Q S W H P C A K D P G W H E Y N R Q S F T A V C M L I M G D E P S A H N I T Q K L W C V Y F R V G D S E N P K Q H T R A C W Y M F L I N G W A Y S F K R H D P E T C M Q V I L E D G K H P W S Y N F T Q C V A R L I M A D E K P N H Y T G S R Q W F V I M L C L G D E P S H K A N Q R W T Y C V F M I V G D K E N H S Q W P R T A Y C M F L I R P W G F Y D C E S A H N V T M I K L Q Q G D K W S H Y N E T F P A I V C L M R G K D Y H E W N P R F T M Q I S L A V C L P G D N S H E T Q A K C R Y W V I F M A E D K H R N Q W T P Y S F G V L I M C K G D H P S N A E Y T W Q C F V I L M R V G E D H K S P Q N R T Y A W M F C I L A D W E H Q N Y P K G T R S F L V M I C Y D E P K N H Q S T V A G C R W M I L F V E G H D K W Q A F Y P N S L M C R T I Y D P E N Q G H K S T C A V I L M W R F K D W Y E F H P G N R T Q I S C M A V L P H Y E L N W Q F D I V K S G M A T C R N D W Y H G P S E K A R F I C Q T L V M N G H K Y F W I A P E M S Q D V R L C T T W H G Y F K M Q P I V L C N R A D E S H D E G P R K S T N A Q W C V I F L Y M E D K R G W P Q S C N H T Y F V L A I M Q W Y D H K G T E V S N F M R I C P L A H D K G W P T N C Y V E Q S I F M R A L L G P D K H S E N T A R Q W Y C F V M I R G D P W E S T N Y H C A K F Q V M I L K D W H Y Q G E P T S N F M V C L I A R S D E H P G Q K N Y T R W M V F I L C A E D G H P R S K F T Y W N C A V I Q M L A W H Y F E P T Q I L D V C K M N G R S Q G P D K Y W H S T F E N V I C L A M R A K E D H R Q P N T Y W G S V M I F L C K D P G E W A H S N Y C F T Q V I M R L


    Segment 3 from 129th residue to 129th residue.
    Download the score file which describes the possibility of mutation for each residue.
    Download all the predicted ddGs for each residue mutanted to other 19 possible amino acids.

    129 0 2.16 4.32 6.47 8.63 10.79 12.95 15.11 17.26 19.42 21.58 K D W E G P Y H C N F S T Q A V M L I R



    • Please cite the following articles when you use the STRUM server:
    1. Lijun Quan, Qiang Lv, and Yang Zhang, STRUM: Structure-based stability change prediction upon single-point mutation, Bioinformatics, Submitted (2015)