Threpp TPP9

Threpp results for TPP9

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>chain A (99 residues) [Download] MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQR PGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE
>chain B (90 residues) [Download] MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTG RNPQTGKEITIAAAKVPSFRAGKALKDAVN

Top 20 dimer threading templates

Rank

PDB
hit

BioUnit
Num

Chain
A

Chain
B

Threpp
score

Iden

Cov

Norm.
Prob.

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180

                   |                   |                   |                   |                   |                   |                   |                   |                   |

MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDEmnksqlidkiaagadiskaaagraldaiiasvteslkegddvalvgfgtfavkeraartgrnpqtgkeitiaaakvpsfragkalkdavn

4qjn

16.721

0.446

0.889

100.0

Template1

--MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQLIGFGNFEVRERAARGRNPQT-GKEIDIPASKVPAFKAGKALKDAVK-------mnktdlinavaeqadltkkeagsavdavfesiqnslakgekvqligfgnfevreraarg-----------ipaskvpafkagkalkdavk

4qjn

16.684

0.439

0.915

100.0

Template2

--MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQLIGFGNFEVRERAARKGRN---PQGIDIPASKVPAFKAGKALKDAVK-------mnktdlinavaeqadltkkeagsavdavfesiqnslakgekvqligfgnfevrerargnpq----tgkidipaskvpafkagkalkdavk

1p51

16.184

0.385

0.963

100.0

Template3

--MNKGELVDAVAEKASVTKKQADAVLTAALETIIEAVSSGDKVTLVGFGSFESRERKAREGRNPKTNEKMEIPATRVPAFSAGKLFREKVAPP-----mnkgelvdavaekasvtkkqadavltaaletiieavssgdkvtlvgfgsfesrerkaregrnpktnekmeipatrvpafsagklfrekva

2np2

16.155

0.317

0.952

100.0

Template4

--VTKSDIVDQIALNIKLEKKYIRLVIDAFFEELKSNLCSNNVIEFRSFGTFEVRKRKGRLARNQT-GEYVKVLDHHVAYFRPGKDLKERVWG------vtksdivdqialniklekkyirlvidaffeelksnlcsnnviefrsfgtfevrkrkgrnarnpqtgeyvkvldhhvayfrpgkdlkervw

1p51

16.119

0.385

0.963

100.0

Template5

--MNKGELVDAVAEKASVTKKQADAVLTAALETIIEAVSSGDKVTLVGFGSFESRERKAREGRNPKTNEKMEIPATRVPAFSAGKLFREKVAPP-----mnkgelvdavaekasvtkkqadavltaaletiieavssgdkvtlvgfgsfesrerkaregrnpktnekmeipatrvpafsagklfrekva

2np2

16.099

0.317

0.952

100.0

Template6

--VTKSDIVDQIALNIKLEKKYIRLVIDAFFEELKSNLCSNNVIEFRSFGTFEVRKRKGRLARNQT-GEYVKVLDHHVAYFRPGKDLKERVWG------vtksdivdqialniklekkyirlvidaffeelksnlcsnnviefrsfgtfevrkrkgrnarnpqtgeyvkvldhhvayfrpgkdlkervw

1mul

15.655

0.480

0.804

99.9

Template7

--MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRA-E------------A-AANVPAFVSGKALKDAVK-------mnktqlidviaekaelsktqakaalestlaaiteslkegdavqlvgfgtfkvnhrae--------------aaanvpafvsgkalkdavk

1mul

15.655

0.480

0.804

99.9

Template8

--MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRA-E------------A-AANVPAFVSGKALKDAVK-------mnktqlidviaekaelsktqakaalestlaaiteslkegdavqlvgfgtfkvnhrae--------------aaanvpafvsgkalkdavk

4p3v

15.619

0.632

0.762

99.9

Template9

--MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKER------------------AKVPSFRAGKALKDAVN-------mnksqlidkiaagadiskaaagraldaiiasvteslkegddvalvgfgtfavker------------------akvpsfragkalkdavn

4p3v

15.619

0.632

0.762

99.9

Template10

--MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKER------------------AKVPSFRAGKALKDAVN-------mnksqlidkiaagadiskaaagraldaiiasvteslkegddvalvgfgtfavker------------------akvpsfragkalkdavn

1huu

15.012

0.475

0.847

99.9

Template11

--MNKTELINAVAETSGLSKKDATKAVDAVFDSITEALRKGDKVQLIGFGNFEVRERAARM----------EIPASKVPAFKPGKALKDAVK-------mnktelinavaetsglskkdatkavdavfdsitealrkgdkvqligfgnfevreraarm----------eipaskvpafkpgkalkdavk

1huu

15.012

0.475

0.847

99.9

Template12

--MNKTELINAVAETSGLSKKDATKAVDAVFDSITEALRKGDKVQLIGFGNFEVRERAARM----------EIPASKVPAFKPGKALKDAVK-------mnktelinavaetsglskkdatkavdavfdsitealrkgdkvqligfgnfevreraarm----------eipaskvpafkpgkalkdavk

2o97

14.877

0.447

0.746

99.9

Template13

--MNKSQLIDKIAAGAD-SKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKER------------------AKVPSFRAGKALKDAVN-------mnktqlidviaekaelsktqakaalestlaaiteslkegdavqlvgfgtfkvnh--------------------nvpafvsgkalkdavk

2o97

14.838

0.660

0.746

99.9

Template14

--MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNH--------------------NVPAFVSGKALKDAVK-------mnksqlidkiaaga-dskaaagraldaiiasvteslkegddvalvgfgtfavker------------------akvpsfragkalkdavn

4pt4

14.486

0.366

0.968

99.9

Template15

--MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFRPGAQFKAVVSGAQ----mnkaelidvltqklgsdrrqataavenvvdtivravhkgdsvtitgfgvfeqrrraarvarnprtgetvkvkptsvpafrpgaqfkavvs

4pt4

14.479

0.366

0.968

99.9

Template16

--MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFRPGAQFKAVVSGAQ----mnkaelidvltqklgsdrrqataavenvvdtivravhkgdsvtitgfgvfeqrrraarvarnprtgetvkvkptsvpafrpgaqfkavvs

1ihf

14.296

0.308

0.963

99.9

Template17

--MTKSELIERLATQQSIPAKTVEDAVKEMLEHMASTLAQGERIEIRGFGSFSLHYRAPRTGRNPT-GDKVELEGKYVPHFKPGKELRDRANIYG----ltkaemseylfdklglskrdakelvelffeeirralengeqvklsgfgnfdlrdknqrpgrnpktgedipitarrvvtfrpgqklksrve

1ihf

14.089

0.667

0.952

100.0

Template18

--LTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQRPGRNKT-GEDIPITARRVVTFRPGQKLKSRVEN------mtkselierlatqqsipaktvedavkemlehmastlaqgerieirgfgsfslhyraprtgrnpktgdkvelegkyvphfkpgkelrdran

3rhi

13.798

0.394

0.873

99.9

Template19

--MNKTELIKNVAQNAEISQKEATVVVQTVVESITNTLAAGEKVQLIGFGTFEVRERAARTG--QT-GEEMQIAASKVPAFKAGKELKEAVK----------teliknvaqnaeisqkeatvvvqtvvesitntlaagekvqligfgtfevrerargr---------mqiaaskvpafkagkelkeavk

3rhi

13.773

0.406

0.899

99.9

Template20

-----TELIKNVAQNAEISQKEATVVVQTVVESITNTLAAGEKVQLIGFGTFEVRERAARGRNP---QTEMQIAASKVPAFKAGKELKEAVK-------mnkteliknvaqnaeisqkeatvvvqtvvesitntlaagekvqligfgtfevreraartgq----tgemqiaaskvpafkagkelkeavk

(a)	Templates are ranked in descending order of ThreppScore of dimer threading.
(b)	BioUnit Num is the biological assembly (i.e. biounit) number.
(c)	Chain A and Chain B are the PDB chains in biological assembly files that aligns the first and second query sequence, respectively.
(d)	ThreppScore, also known as SPRING-score, is a combination of monomeric threading Z-score, interface contact statistical potential, and TM-align match between monomer-to-dimer templates.
(e)	Iden is the sequence identity of the templates in the threading aligned region with the query sequence.
(f)	Cov is the coverage of threading alignment. It is equal to the number of aligned residues divided by the length of two query proteins.
(g)	Norm Prob is the percentage probability of correct dimer template identified.
(h)	Download alignment provides the 3D coordinates of template aligned regions.
(f)	Template residues identical to query sequence are highlighted in color. Upper and lower case letters denote residues from first and second chain, respectively.
(g)	The full template table is available here.

Top 20 structure models

Click to view	Rank^a	Download^b
	1	model1.pdb.gz
	2	model2.pdb.gz
	3	model3.pdb.gz
	4	model4.pdb.gz
	5	model5.pdb.gz
	6	model6.pdb.gz
	7	model7.pdb.gz
	8	model8.pdb.gz
	9	model9.pdb.gz
	10	model10.pdb.gz
	11	model11.pdb.gz
	12	model12.pdb.gz
	13	model13.pdb.gz
	14	model14.pdb.gz
	15	model15.pdb.gz
	16	model16.pdb.gz
	17	model17.pdb.gz
	18	model18.pdb.gz
	19	model19.pdb.gz
	20	model20.pdb.gz

(a)	Threpp models are ranked in the same order as the respective dimer templates, as listed here.
(b)	Download structure models.

Combination with high-throughput experiment (HTE)

#	chain A (seqID) ^a	chain A (seqID) ^b	PPI ^c	Dataset source
0	2o97A/1_0_0 (0.253)	2o97A/1_1_7 (0.789)	Y	Dimer theading
1	b1712 (100.0%)	b0440 (100.0%)	Y	Hu et al
2	b1712 (100.0%)	b0440 (100.0%)	N	Rajagopala et al
3	b1712 (100.0%)	b0440 (100.0%)	N	Arifuzzaman et al
4	b1712 (100.0%)	b0440 (100.0%)	Y	Butland et al

	Likelihood ratio of interaction = 8.16 ≥ 1.87, the protein pair is predicted to interact.
(a)	b-number of chain A in the HTE dataset, and the percentage sequence identity between query chain and the sequence used in HTE.
(b)	b-number of chain B in the HTE dataset, and the percentage sequence identity between query chain and the sequence used in HTE.
(c)	Whether the protein pair is found to interact in the HTE: Y for interacting pair; N for non-interacting pair; NA for a protein pair not included in the HTE.

[Click result.zip to download all results on this page]

References:

Weikang Gong, Aysam Guerler, Chengxin Zhang, Elisa Warner, Chunhua Li, Yang Zhang. Integrating Multimeric Threading With High-throughput Experiments for Structural Interactome of Escherichia coli . Journal of Molecular Biology, 433: 166944 (2021). [PDF] [Supporting Information]