Design Strategy: The default setting of EvoDesign for Interface Design is to redesign the entire sequence of the scaffold chain. The rationale behind designing non-interface residues is that introducing mutations in the interface may have destabilizing effects on the whole protein or lead to suboptimal packing. However, for some large complexes with specific folding architectures such as antibody-antigen complexes, it might be beneficial to focus the design only on the interface regions. Thus, for Interface Design, users are given an additional option to either redesign the entire scaffold protein or to redesign only its interface residues, which are defined as residues within 5 Å of the opposite chain.