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I-TASSER results for job id S693375

(Click on S693375_results.tar.bz2 to download the tarball file including all modeling results listed on this page. Click on Annotation of I-TASSER Output to read the instructions for how to interpret the results on this page. Model results are kept on the server for 60 days, there is no way to retrieve the modeling data older than 2 months)

  Submitted Sequence in FASTA format

>protein
MGDNHPIGPIGAVGAAPGDGVQGGADAIPVRRHQLRAVQRHQVDDFDIQIPAHGSGELRT
VTRRSAHSVGARAGRDRSSGGDNGEAGHGGIISHPCHRHPDVGTGTKLRDDGGVSDEPTG
SADPLTTAGETGVTEEPKVSAPEPPPRRLRLLLSVAAVVLALDVVTKVLAVKLLPPGQPV
SIIGDTVTWTLVRNSGAAFSMATGYTWVLTLIATGVVVGIFWMGRRLVSPWWAVGLGMIL
GGAMGNLVDRFFRAPGPLRGHVVDFFSVGWWPVFNVADPSVVGGAILLVILSIFGFDFDT
VGRRKADER

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |         
MGDNHPIGPIGAVGAAPGDGVQGGADAIPVRRHQLRAVQRHQVDDFDIQIPAHGSGELRTVTRRSAHSVGARAGRDRSSGGDNGEAGHGGIISHPCHRHPDVGTGTKLRDDGGVSDEPTGSADPLTTAGETGVTEEPKVSAPEPPPRRLRLLLSVAAVVLALDVVTKVLAVKLLPPGQPVSIIGDTVTWTLVRNSGAAFSMATGYTWVLTLIATGVVVGIFWMGRRLVSPWWAVGLGMILGGAMGNLVDRFFRAPGPLRGHVVDFFSVGWWPVFNVADPSVVGGAILLVILSIFGFDFDTVGRRKADER
PredictionCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCSSCCCCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSSSSSCCSSSSSSSSCCCCSSCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCSSSSSSSCCCCCSSSHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
Conf.Score998887775311345888765477554311488888888742663223324678752034203677886545799998888876567675446855578775667777766788889889999877778887777765557777630899999999999999999999999866579848975886899998518815424344179999999999999999988627889999999986101132766274568865447987153588756677999999999999999997678431100121259
H:Helix; S:Strand; C:Coil

  Predicted Solvent Accessibility

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |         
MGDNHPIGPIGAVGAAPGDGVQGGADAIPVRRHQLRAVQRHQVDDFDIQIPAHGSGELRTVTRRSAHSVGARAGRDRSSGGDNGEAGHGGIISHPCHRHPDVGTGTKLRDDGGVSDEPTGSADPLTTAGETGVTEEPKVSAPEPPPRRLRLLLSVAAVVLALDVVTKVLAVKLLPPGQPVSIIGDTVTWTLVRNSGAAFSMATGYTWVLTLIATGVVVGIFWMGRRLVSPWWAVGLGMILGGAMGNLVDRFFRAPGPLRGHVVDFFSVGWWPVFNVADPSVVGGAILLVILSIFGFDFDTVGRRKADER
Prediction856622111010112132322422242131343314314434045141302332433133134334443335435743432534332442324233434144454434446443456444555434444444446555454443332101101110120020023002000320444331201132010101201100000234322200120222011010002223430100000002111310000001134422200000001133010000000000100000000001235376465545778
Values range from 0 (buried residue) to 9 (highly exposed residue)

   Predicted normalized B-factor

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. Click here to read more about predicted normalized B-factor)


  Top 10 threading templates used by I-TASSER

(I-TASSER modeling starts from the structure templates identified by LOMETS from the PDB library. LOMETS is a meta-server threading approach containing multiple threading programs, where each threading program can generate tens of thousands of template alignments. I-TASSER only uses the templates of the highest significance in the threading alignments, the significance of which are measured by the Z-score, i.e. the difference between the raw and average scores in the unit of standard deviation. The templates in this section are the 10 best templates selected from the LOMETS threading programs. Usually, one template of the highest Z-score is selected from each threading program, where the threading programs are sorted by the average performance in the large-scale benchmark test experiments.)

Rank PDB
Hit
Iden1Iden2CovNorm.
Z-score
Download
Align.
                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |         
Sec.Str
Seq
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCSSCCCCCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSSSSSCCSSSSSSSSCCCCSSCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCSSSSSSSCCCCCSSSHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
MGDNHPIGPIGAVGAAPGDGVQGGADAIPVRRHQLRAVQRHQVDDFDIQIPAHGSGELRTVTRRSAHSVGARAGRDRSSGGDNGEAGHGGIISHPCHRHPDVGTGTKLRDDGGVSDEPTGSADPLTTAGETGVTEEPKVSAPEPPPRRLRLLLSVAAVVLALDVVTKVLAVKLLPPGQPVSIIGDTVTWTLVRNSGAAFSMATGYTWVLTLIATGVVVGIFWMGRRLVSPWWAVGLGMILGGAMGNLVDRFFRAPGPLRGHVVDFFSVGWWPVFNVADPSVVGGAILLVILSIFGFDFDTVGRRKADER
16rypA 0.25 0.16 0.51 2.18Download ---------------------------------------------------------------------------------------------------------------------------------------------HMHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDANLFMQVAISLLFAGALGNFIDRV------LTGEVVDFIDTNDFPIFNIADSSLTIGVILIIIALLKDTSNKKEKEVK----
27wooD 0.07 0.14 0.79 1.34Download IKPVYNRYSVSTVSSLLS--------------------------------------------KPTLSTATTNLQQSITGFSKPSPANKEDFDLDDVIGRHVFMTFTDNRVTSHAFISSSDPITPSINNLKSDEISQNRNIISKVSISKDCIEYYLSSINILNEFFITYGDSISQISAPY------------VLANNSNGRVIDKTEEVANQAESIAINAMIKMVQSIKEGLSFLNVLYE---------ESEVEGFDNQYLGFKDIISFVSLDVLDFKDLFATKSLIREILLSIINRNITKGASIEYCSA
36ryo 0.26 0.15 0.48 7.77Download ---------------------------------------------------------------------------------------------------------------------------------------------HMHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKQYNLFMQVAISLLFAGALGNFIDRVL------TGEVVDFIDTNDFPIFNIADSSLTIGVILIIIALLKDT-------------
47rjtA 0.09 0.28 1.00 1.08Download TMRSYKPTPEMSQWQTDYMRGTGMAFNALTFPEAAELCFSKLKLLLLAIEVRQEDTRESTLAINPGPKVKIENATFYYCKNCHANVSDVRQIKKCKCRPEPRKFDSTGMFHWCPDRPLNDCLQDRSQASASGLRNHADAASPLIGLRNLVMPLRASTIIVGNLDYLHREWKTLQNFPKLSILPGSMCVIVSAKDRNMEDPNLVDKEAILCSLNIKSVLDSLMSTSYFNDNALTLIRTLITGGATPELEQILAEGAGMRDRCRVAQISLFDGPLAHYGELFVYAGILCIGLYRFRDTNESVRSPSSKYKQ
56ryo 0.26 0.15 0.48 5.68Download ---------------------------------------------------------------------------------------------------------------------------------------------HMHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKQYNLFMQVAISLLFAGALGNFIDRVLT------GEVVDFIDTNDFPIFNIADSSLTIGVILIIIALLKDT-------------
67egmB 0.09 0.19 0.94 1.05Download NKPIFLFAPELGAIADSNLKISLVKYPELLDSLAILGMNLLSNLS--------------------QNVVPYHRNTSDYYYEDQHDKMVDKIFEKVNNNSPAVKQWDLLPEPIRFLPNQFPLKIHRTPYLTDDPFTKINTRGAEDPKVLINDQLSTISMILYLKRFISDLLWLVLIHPENFTCISHLLEIAQPKLESLTFNEFQLQWGKYQTFGVDILANYFKSILLNNHKDKKLLRRLLNLYNRHNLLNDVILPFNFNKNLPFVWLSSEENIGSGLLKLSINISCVQLIFENCLNNDPEILKKIASNLE
76ryoA 0.26 0.15 0.48 1.63Download -----------------------------------------------------------------------------------------------------------------------------------------------HKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDANLFMQVAISLLFAGALGNFIDRVLT------GEVVDFIDTNIFPIFNIADSSLTIGVILIIIALLKDT-------------
87vdvQ 0.07 0.25 1.00 1.02Download GLRGSPFKKIVEEFNFPRSCSNAAFALKQYYLRYLEKYEKVHHFGEDDDEVPAIPSSYNYQQHSVSDYLRQSYGLSMDFNSPNDYNESKHVMQLEKDPKIITLLLANAGVFDDTLGSFSTVFGEEWKEKTDRDSLFHPPRKLGINDIEGQRVLQIAVILRNLEGNVKLLFLLLSAHSHFISLRQLGLDTLGNIAAELLLDPVDFKTTHLMFHTVFLKMRGMEILGNLCKVISTLEVLYMLTEMGDVACTKIAKVEKSIEHIVEIDSEKTDEKEGPITKHIRLTAALILKLSVLAISNMEASSTLAKNFT
96ryo 0.26 0.15 0.47 8.30Download -------------------------------------------------------------------------------------------------------------------------------------------------KYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKQYNLFMQVAISLLFAGALGNFIDRVL------TGEVVDFIDTNDFPIFNIADSSLTIGVILIIIALLKD--------------
107b5pB 0.13 0.30 0.96 0.79Download VSVEKSSSFLMVVGVINTDG---TMTQEDISDYVADAISRGDVQLFGSQLNKFQLTPVDVIKAQNAQVAAGQLGGTPPVKGQQLNAIAQTRLTSTKILLKVNQDGSELGGENYDNGQPLATGANARAELAKMEPFFPSGLKIVYTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIVVLLFSINTLTMFGMLA--IGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQGALVGIAMVLSAVVGGSTGAI--------YRQFSITIVSAMALSVLVAALCATMLKFNRMFEKSTHHY
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: FFAS-3D   2: PROSPECT2   3: HHSEARCH2   4: PROSPECT2   5: HHSEARCH I   6: PROSPECT2   7: Neff-PPAS   8: PROSPECT2   9: HHSEARCH   10: SPARKS-X   

   Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of a higher value signifies a model with a higher confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated; this is usually an indication that the models have a good quality because of the converged simulations.)
    (By right-click on the images, you can export image file or change the configurations, e.g. modifying the background color or stopping the spin of your models)
  • Download Model 1
  • C-score=-3.52 (Read more about C-score)
  • Estimated TM-score = 0.33±0.11
  • Estimated RMSD = 14.9±3.6Å

  • Download Model 2
  • C-score = -4.07

  • Download Model 3
  • C-score = -4.23

  • Download Model 4
  • C-score = -3.91

  • Download Model 5
  • C-score = -3.18


  Proteins structurally close to the target in the PDB (as identified by TM-align)

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)


Top 10 Identified stuctural analogs in PDB

Click
to view
RankPDB HitTM-scoreRMSDaIDENaCovAlignment
16ryoA0.471 1.030.2550.482Download
25dirA0.446 1.920.3840.469Download
37pw4A0.429 5.240.0470.621Download
42rnpC0.423 6.140.0040.680Download
51q9iA0.415 6.210.0590.676Download
61d4cA0.413 6.220.0740.680Download
73epsA0.412 6.400.0540.693Download
86r9tA0.407 5.410.0340.608Download
96eojA0.407 6.840.0490.731Download
106xkwn0.406 5.260.0490.592Download

(a)Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.


  Predicted function using COFACTOR and COACH

(This section reports biological annotations of the target protein by COFACTOR and COACH based on the I-TASSER structure prediction. While COFACTOR deduces protein functions (ligand-binding sites, EC and GO) using structure comparison and protein-protein networks, COACH is a meta-server approach that combines multiple function annotation results (on ligand-binding sites) from the COFACTOR, TM-SITE and S-SITE programs.)

  Ligand binding sites


Click
to view
RankC-scoreCluster
size
PDB
Hit
Lig
Name
Download
Complex
Ligand Binding Site Residues
10.27 8 5dirD MLE Rep, Mult 209,213,216,243,246,250,281
20.25 6 5dirA SER Rep, Mult 194,250,262,264,275,278
30.13 4 5dirA IIL Rep, Mult 246,250,278,281
40.09 3 5dirC ALO Rep, Mult 194,196,199,209
50.03 1 5dirB 5BV Rep, Mult 209,212,250


Download the residue-specific ligand binding probability, which is estimated by SVM.
Download the all possible binding ligands and detailed prediction summary.
Download the templates clustering results.
(a)C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)Cluster size is the total number of templates in a cluster.
(c)Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column.
Mult is the complex structures with all potential binding ligands in the cluster.

  Enzyme Commission (EC) numbers and active sites


Click
to view
RankCscoreECPDB
Hit
TM-scoreRMSDaIDENaCovEC NumberActive Site Residues
10.1072uxwA0.396 6.480.0650.670 1.3.99.-  241
20.0651d4cA0.413 6.220.0740.680 1.3.99.1  NA
30.0651p1jA0.399 6.220.0550.637 5.5.1.4  NA
40.0651e39A0.323 6.470.0430.540 1.3.99.1  31
50.0653epsA0.412 6.400.0540.693 2.7.11.5  168,278

 Click on the radio buttons to visualize predicted active site residues.
(a)CscoreEC is the confidence score for the EC number prediction. CscoreEC values range in between [0-1];
where a higher score indicates a more reliable EC number prediction.
(b)TM-score is a measure of global structural similarity between query and template protein.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided
by length of the query protein.

  Gene Ontology (GO) terms
Top 10 homologous GO templates in PDB 
RankCscoreGOTM-scoreRMSDaIDENaCovPDB HitAssociated GO Terms
1 0.060.4130 6.22 0.07 0.681d4cA GO:0000104 GO:0009055 GO:0016491 GO:0055114
2 0.060.3710 6.50 0.04 0.641jkiA GO:0006021 GO:0005488 GO:0004512 GO:0005515 GO:0042802 GO:0008654 GO:0005737 GO:0016853
3 0.060.3225 6.47 0.04 0.541e39A GO:0042597 GO:0006810 GO:0055114 GO:0009061 GO:0046872 GO:0019645 GO:0009055 GO:0030288 GO:0022900 GO:0016491 GO:0000104 GO:0005622 GO:0008270
4 0.060.4044 6.29 0.07 0.673hazA GO:0000166 GO:0055114 GO:0016491 GO:0003842 GO:0004657 GO:0006537 GO:0006561 GO:0006562 GO:0008152
5 0.060.4121 6.40 0.05 0.693epsA GO:0000166 GO:0016791 GO:0005524 GO:0006099 GO:0005737 GO:0006097 GO:0004721 GO:0016311 GO:0016740 GO:0008772 GO:0016301 GO:0016310 GO:0006006 GO:0016787
6 0.060.3953 5.90 0.06 0.613afeA GO:0003995 GO:0016627 GO:0006629 GO:0008152 GO:0008202 GO:0055114 GO:0016491 GO:0005886 GO:0050660 GO:0016042 GO:0004497 GO:0019439 GO:0044117
7 0.060.4002 5.74 0.05 0.621is2A GO:0016491 GO:0005777 GO:0033540 GO:0055114 GO:0006091 GO:0005504 GO:0006631 GO:0019395 GO:0003997 GO:0006629 GO:0006693 GO:0016401 GO:0003995 GO:0006635 GO:0008152 GO:0016627 GO:0050660
8 0.060.3907 6.46 0.05 0.632ot4A GO:0022900 GO:0046872 GO:0006810 GO:0016491 GO:0055114 GO:0006807
9 0.060.3875 6.15 0.05 0.631j0mA GO:0003824 GO:0005576 GO:0005975 GO:0016829 GO:0016837 GO:0030246
10 0.060.3958 6.48 0.07 0.672uxwA GO:0006635 GO:0005759 GO:0006631 GO:0003995 GO:0044255 GO:0016491 GO:0005739 GO:0015980 GO:0006629 GO:0046322 GO:0016020 GO:0017099 GO:0033539 GO:0005743 GO:0042760 GO:0000062 GO:0004466 GO:0055114 GO:0050660 GO:0090181 GO:0042645 GO:0045717 GO:0001659 GO:0008152 GO:0016627


Consensus prediction of GO terms
 
Molecular Function GO:0009055 GO:0000104 GO:0004657 GO:0008772 GO:0042802 GO:0004721 GO:0003842 GO:0004512 GO:0008270 GO:0005524
GO-Score 0.12 0.12 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07
Biological Process GO:0006006 GO:0006099 GO:0019645 GO:0016311 GO:0006810 GO:0006537 GO:0006021 GO:0016310 GO:0006562 GO:0006097
GO-Score 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07
Cellular Component GO:0005737 GO:0030288
GO-Score 0.12 0.07

(a)CscoreGO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)TM-score is a measure of global structural similarity between query and template protein.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on CscoreGO of the template.


[Click on S693375_results.tar.bz2 to download the tarball file including all modeling results listed on this page]



Please cite the following articles when you use the I-TASSER server:
  • Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. Cell Reports Methods, 1: 100014 (2021).
  • Chengxin Zhang, Peter L. Freddolino, and Yang Zhang. COFACTOR: improved protein function prediction by combining structure, sequence and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017).
  • Jianyi Yang, Yang Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.