Search found 3 matches

by UnknownUser
Tue Jun 29, 2021 11:51 am
Forum: Main Forum
Topic: Distance restraints for I-TASSER Problem
Replies: 3
Views: 1721

Re: Distance restraints for I-TASSER Problem

One possible reason is that the weight of your provided distance constraint is less than that of the I-TASSER potential, since you just provided one residue pair. Thanks for your quick reply xiaogenz, I have just posted the first restraint (/line) to show the formatting, but I submitted 23 restrain...
by UnknownUser
Fri Jun 25, 2021 6:33 am
Forum: Main Forum
Topic: Distance restraints for I-TASSER Problem
Replies: 3
Views: 1721

Re: Distance restraints for I-TASSER Problem

Thanks for your quick reply xiaogenz, I have just posted the first restraint (/line) to show the formatting, but I submitted 23 restraints all with the same distance and all between alpha carbons like this: DIST 216 CA 304 CA 9.8 DIST 79 CA 154 CA 9.8 DIST 118 CA 196 CA 9.8 DIST 59 CA 240 CA 9.8 DIS...
by UnknownUser
Wed Jun 23, 2021 11:10 am
Forum: Main Forum
Topic: Distance restraints for I-TASSER Problem
Replies: 3
Views: 1721

Distance restraints for I-TASSER Problem

I have been trying to use the tool to predict the structure of a protein with distance restraints from in-vitro crosslinking data. I have tried to follow the instructions in https://zhanglab.dcmb.med.umich.edu/I-TASSER/restraint.html for preparing the restraints .txt file and submitted several itera...