Distance restraints for I-TASSER Problem
Posted: Wed Jun 23, 2021 11:10 am
I have been trying to use the tool to predict the structure of a protein with distance restraints from in-vitro crosslinking data. I have tried to follow the instructions in https://zhanglab.dcmb.med.umich.edu/I-T ... raint.html for preparing the restraints .txt file and submitted several iterations. None of them seem to work and the restraints are not met in the output. Could anybody instruct us on how to submit the restraint .txt file correctly?
The pertinent job ID's are the following, see the first line in the restraint .txt file for clarification:
-S623936 (Tab-delimited)
DIST 216 CA 304 CA 9.8
-S624319 (Spaces identical to example)
DIST 216 CA 304 CA 9.8
-S624780 (Single-space-delimited)
DIST 216 CA 304 CA 9.8
Copy and pasted template from : https://zhanglab.dcmb.med.umich.edu/I-T ... raint.html
DIST 124 CA 84 CA 17.4
Note that these may look different dispayed on this post but if copied onto a txt document they are displayed properly.
Thanks for your help in advance!
The pertinent job ID's are the following, see the first line in the restraint .txt file for clarification:
-S623936 (Tab-delimited)
DIST 216 CA 304 CA 9.8
-S624319 (Spaces identical to example)
DIST 216 CA 304 CA 9.8
-S624780 (Single-space-delimited)
DIST 216 CA 304 CA 9.8
Copy and pasted template from : https://zhanglab.dcmb.med.umich.edu/I-T ... raint.html
DIST 124 CA 84 CA 17.4
Note that these may look different dispayed on this post but if copied onto a txt document they are displayed properly.
Thanks for your help in advance!