Generate Protein

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Moderator: robpearc

Edward Pullen
Posts: 3
Joined: Fri Jul 09, 2021 5:23 am

Generate Protein

Post by Edward Pullen »

Hi Everyone,
I am using I-TASSER to generate protein models of Uniprot sequence and the the results showing TM-score 0.29 , RMSD 15.7 and C-score 3.42 for the top model.
How can I evaluate the generated protein model? Please advise!
robpearc
Posts: 40
Joined: Sun May 02, 2021 5:49 pm

Re: Generate Protein

Post by robpearc »

Generally, a TM-score >=0.5 indicates that a protein has the correct global fold. Thus, in your case, the model seems to be low confidence. However, this is an estimation based on our statistics and may not reflect the actual TM-score.
Edward Pullen
Posts: 3
Joined: Fri Jul 09, 2021 5:23 am

Re: Generate Protein

Post by Edward Pullen »

Please advise how can I improve TM-Score to 0.5?
xiaogenz
Posts: 73
Joined: Sun Apr 25, 2021 12:02 am

Re: Generate Protein

Post by xiaogenz »

You can try to provide the template and distance restraints.
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