Help with CRISPR Base Editing Efficiency in Cell Cultures

[cevolution]

Hello everyone,

I’ve been working with CRISPR base editing in mammalian cell cultures, but I’ve been facing challenges with editing efficiency. Despite following the protocol closely, the success rate for desired mutations remains lower than expected. I’ve optimized transfection conditions and verified sgRNA design, but I’m still not seeing significant improvements.
hill climb racing
Does anyone have tips or suggestions for improving base editing efficiency in cell cultures? Are there specific variables (like timing or reagent choices) that you’ve found critical in boosting success rates?

Thanks in advance for any advice!