Regarding Modelling of Pentamer Nanobody

[Ahbab Ali]

Good evening sir\ma'am
I hope this message finds you well. Currently, I am working on a homology modeling of a homo-pentameric nanobody project which is part of my dissertation. My query is, can I make a homo-pentamer of a Nanobody, for which templates are not available on PDB by the I-TASSER web server? if yes! then, please share the protocol or steps it would be helpful for me.
Thank you
Ahbab Ali
M.S.(Pharm.) Biotechnology
National Institute of Pharmaceutical Education and Research

[jlspzw]

Dear user,

You can try our most cutting-edge method https://zhanggroup.org/D-I-TASSER/

Best
IT Team

[peoplebreast]

Good evening sir\ma'am
I hope this message finds you well. Currently, I am working on a homology modeling of a homo-pentameric nanobody project which is part of my dissertation. My query is, can I make a homo-pentamer of a Nanobody, for which templates are not available on PDB by the I-TASSER web server? if yes! then, please share the protocol or steps it would be helpful for me.
Thank you
Ahbab Ali
M.S.(Pharm.) Biotechnology
National Institute of Pharmaceutical Education and Research

Yes, I-TASSER models only monomeric structures. For a homo-pentamer nanobody, first use I-TASSER to model the monomer, then use tools like SymmDock, ClusPro, or Rosetta SymDock to assemble the pentamer based on symmetry.