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BioLiP

Structure of PDB 4lgt Chain A Binding Site BS01

Receptor Information
>4lgt Chain A (length=251) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKI
RIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRG
ARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDD
AKLRDLSRGVQLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLW
EAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLRELVELPPET
S
Ligand information
>4lgt Chain E (length=21) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
agaacgucgugagacaguucg
.<.<<<<<....>>>.>>.>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB4lgt The mechanism of pseudouridine synthases from a covalent complex with RNA, and alternate specificity for U2605 versus U2604 between close homologs.
Resolution1.3 Å
Binding residue
(original residue number in PDB)		K4 Q6 K7 S16 R17 R18 E21 L38 G39 D40 R41 L79 T81 R82 R88 R102 G107 R108 L109 D110 V111 G123 N127 H131 P132 S133 E135 R138 Y140 G191 N193 R194 V196 L210 R212 Y215
Binding residue
(residue number reindexed from 1)		K4 Q6 K7 S16 R17 R18 E21 L38 G39 D40 R41 L79 T81 R82 R88 R102 G107 R108 L109 D110 V111 G123 N127 H131 P132 S133 E135 R138 Y140 G191 N193 R194 V196 L210 R212 Y215
Enzymatic activity
Enzyme Commision number 5.4.99.22: 23S rRNA pseudouridine(2605) synthase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity
GO:0016866 intramolecular transferase activity
Biological Process
GO:0001522 pseudouridine synthesis
GO:0009451 RNA modification

View graph for
Molecular Function
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Biological Process
External links
PDB RCSB:4lgt, PDBe:4lgt, PDBj:4lgt
PDBsum4lgt
PubMed24214967
UniProtP37765|RLUB_ECOLI Ribosomal large subunit pseudouridine synthase B (Gene Name=rluB)

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