C-I-TASSER (Contact-guided Iterative Threading ASSEmbly Refinement) is a new method extended from I-TASSER for high-accuracy protein structure and function predictions. Starting from a query sequence, C-I-TASSER first generates inter-residue contact maps using multiple deep neural-network predictors, including NeBcon, ResPRE, and TripletRes. It then identifies structural templates from the PDB by multiple threading approach LOMETS, with full-length atomic models assembled by contact-map guided replica-exchange Monte Carlo simulations. Biological functions of the query protein are finally derived from the structure model by COFACTOR. The large-scale benchmark tests showed that C-I-TASSER generates significantly more accurate models than I-TASSER, especially for the sequences that do not have homologous templates in the PDB. The output model of C-I-TASSER server is given by both PDB format and ModelCIF format now. Please report problems and questions at our Discussion Board.
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